Actin fiber alignment

Hey There,

Does anyone have the ability to or have thought to create a module that could give an indication of actin filament alignment… i.e. I work with muscle cells, and one of the first signals that cells are moving into a position to fuse, is alignment.

I am trying to find a way to create an alignment index using either nuclei positions or better yet, actin filament orientation (for fluorescent images).

Does anybody have any suggestions or pre-created modules/programs?